Brain insulin resistance and Alzheimer’s molecular changes induced by Western diet

Background Western diet (WD) is a type of nourishment based on ultra-processed foods, rich in simple sugars and saturated fats. Long-term consumption WD may lead to disruption insulin signaling development resistance. It supposed that resistance probable risk factors for Alzheimer’s disease (AD). The aim this study was verify hypothesis wild mice by checking the effect initiation propagation main neuropathological AD features such as amyloid-β (Aβ) plaques neurofibrillary tangles, which start from entorhinal cortex temporal area, progresses hippocampus, resulting loss memory cognition. Method Males C57BL/6J were fed or standard (SD; CTR). Mice experimental groups (WD CTR) divided into age subgroups 4-, 8-, 12- 16-month-old. In first step, WD-diet dependent analyzed, levels pathway components: p-IRS-1(Ser616), p-Akt(473), p-GSK-3β(Ser9) hippocampus assessed. To analyze further whether WD-derived impairments induce features, p-Tau(Thr231) APP/Aβ analyzed immunoblotting immunofluorescence mouse brain tissue sections hippocampus. Result Entorhinal proved be more sensitive WD-dependent than hippocampus: analysis lysates revealed an increase p-IRS-1(Ser616) levels, indicating under diet. Moreover, change localization cellular compartments fibers nerve cell bodies indicated progressive tauopathy. addition, we also observed age-dependent decrease APP protein correlating with appearance Aβ peptides cytoplasm neurons Conclusion Obtained results suggest diet, inducing abnormalities brain, promotes AD, consider significant modifiable factor additional genetic factors.